Automated Imaging Classroom – Educational Videos

Dover Motion CTO, Kevin McCarthy dives into motion control concepts and calculations for imaging applications.

Microscopy Calculator Walk-Through

Dover Motion Microscopy Calculator

Click the button below for a pdf of the calculations file

Covers key formulas for selecting the optimal imaging sensor and microscope objective for your digital imaging application including sensor size, magnification, field of view, pixel sizes, resolution, depth of field, and numerical aperture.

Depth of Field Explained

Depth of field is an important criteria for selecting the precision and resolution of focusing stages for automated imaging applications. This video explains what depth of field refers to for objective focusing. It also shows how to calculate depth of field using your objective’s numerical aperture and refractive index of air, water, or oil.

Time Delay Integration (TDI) Imaging

TDI scanning is a method to increase instrument throughput when imaging a biological sample or flow cell for DNA Sequencing. This video helps illustrate how Time Delay Integration constant velocity imaging works (also known as TDI). When TDI image sensors are used, images are taken on the fly, while the stage / sample moves in a straight line at constant velocity. TDI scanning imaging is considerably more time efficient, since the sensor is continuously imaging and reading out, with no wasted time spent moving and settling between separate fields of view.

Limitations of Piezo Stages

Piezo stages are a unique class of actuators that can be a valuable tool, though they are often plagued with issues of high cost with limited travel, speeds, and bandwidth. In this video Kevin explores different Piezo actuator technologies and contrasts them to linear motor stages. If you prefer to read the article, the full transcript along with pictures and diagrams is available.

Automated Digital Microscope Concepts for Fluorescence Imaging

Modern life science and diagnostic instruments rely on automated digital microscope technologies. When designing these complex systems, there are a lot of options to consider. In this video, Kevin will explain the XY stage for sample positioning, and Z stage for precision microscope objective focusing motion. Since many applications require fluorescence imaging, we will cover the basic concepts and components that make fluorescence work.

Microscope Calculations Explained: Field Of View, Depth Of Field, Numerical Aperture

When designing automated digital microscopy instruments that include imaging, high resolution images and fast throughput are critical. In this video, Kevin will explain key optical imaging formulas and how they can help optimize your automated digital microscopy imaging application. Topics that will be covered are selecting the right type of sensor and sensor size, magnification, field of view, pixel size, resolution, depth of field, and numerical aperture for digital microscopy.